In present investigation 147 Basidiome of Wood-rotting fungi were collected from various region of Nanded District from that 16 genera, and 20 species were newly identified from study area. All these macro-fungi were identified according to macro-morphological and microscopic feature, most dominating genera were observe Leucocoprinus (3 species), Coriolopsis (2 species), and Inonotus (2 specie).