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VOL. 10, ISSUE 1 (2025)
Effect of cytokinin and silver nitrate on in vitro regeneration of Stereospermum calais L.: An important medicinal forest tree
Authors
Vilas R Parmar
Abstract
Stereospermum calais
L. is a well-known pharmacologically powerful herb reported in convention
medical systems. Micropropagation of Sterepspermum calais was based on
nodal explants grown on a MS medium supplemented by various combinations of
BAP. Induction of multiple shoots with a maximum of 6 shoots and a shoot length
of 2 cm was observed on the MS medium supplemented by 3 mg L-1 BAP
and AgNO3 (1 mg L-1). For root induction, in vitro
generated shoots were treated with various IBA concentrations on a basal MS
medium of ¼ concentration, supplemented by AgNO3 (1 mg L-1).
The maximum number of roots (10) was found on ¼ MS level with 1 mg L-1
IBA and AgNO3 (1 mg L-1). The seedlings produced were
treated with the hardening procedure for acclimatization and transferred to the
soil.
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Pages:46-49
How to cite this article:
Vilas R Parmar "Effect of cytokinin and silver nitrate on <i>in vitro</i> regeneration of <i>Stereospermum calais</i> L.: An important medicinal forest tree". International Journal of Botany Studies, Vol 10, Issue 1, 2025, Pages 46-49
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