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VOL. 10, ISSUE 1 (2025)
Antioxidant, anticoagulant, antiplatelet and anti-eryptotic (protection of RBCs) activities of Acacia sinuata leaves aqueous extract
Authors
Ramesha KL, Sujatha M, Shivakumar V, Devaraja S, Dr. Hemalatha MS
Abstract
Acacia sinuata
is the perennial, woody, large climbing shrub which grows on big trees belongs to the Fabaceae. It has been extensively used in the folk medicine since ancient time
due to its arrays of medicinal qualities as it pacifies ulcer, heart burn, indigestion, constipation, skin
disease, burning sensation, calculi, hemorrhoids, vitilligo, eczema and jaundice. While, none of the studies reported the protective role of Acacia sinuata extract
on oxidative stress induced complications. Thus, present study proposes to
scrutinize the protective role of Acacia sinuata Leaves Aqueous Extract (ASLAE) on oxidative stress induced
RBCs damage and thrombosis. Preliminary qualitative
phytochemical screening revealed the presence of carbohydrates, tannins,
alkaloids, flavonoids, steroids and polyphenols. ASLAE showed 85% of
DPPH scavenging activity with an IC50 value of 6.94 µg/mL.
Interestingly ASLAE significantly (***p < 0.0001) normalized the stress
markers such as Lipid Peroxidation (LPO), Protein Carbonyl Content (PCC), Total
Thiol (TT), endogenous antioxidant enzymes such as Superoxide dismutase (SOD)
and catalase (CAT) in sodium nitrite induced oxidative stress in RBCs. ASLAE
displayed an anticoagulant effect by enhancing normal clotting time in platelet
rich plasma from 120 sec to 800 sec. The anticoagulant effect of ASLAE
was also strengthened by APTT and PT tests. ASLAE was positive for both
APTT and PT test, revealed the identified anti-coagulant effect of ASLAE
owing to intervention in intrinsic and extrinsic pathway of blood coagulation
cascade. ASLAE also repressed both ADP and epinephrine induced platelet
aggregation with an inhibition percentage of 70% & 65% respectively. ASLAE
was non-toxic in nature as it was lacking hemolysis of RBCs and good in
resolving oxidative stress induced pathogenesis and thrombotic disorders.
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Pages:4-11
How to cite this article:
Ramesha KL, Sujatha M, Shivakumar V, Devaraja S, Dr. Hemalatha MS "Antioxidant, anticoagulant, antiplatelet and anti-eryptotic (protection of RBCs) activities of <i>Acacia sinuata</i> leaves aqueous extract". International Journal of Botany Studies, Vol 10, Issue 1, 2025, Pages 4-11
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