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VOL. 10, ISSUE 10 (2025)
Comparative analysis of root nodulation and rhizobial diversity in legume species growing in Amer Region, Jaipur
Authors
Amit Yadav, Sonam Choudhary, Indu Singh Sankhla
Abstract
Legume–rhizobia symbiosis plays a crucial role in sustainable nitrogen
management, particularly in diverse ecosystems where host plants exhibit
variable nodulation responses. In the present study, five leguminous species
namely Alysicarpus vaginalis, Indigofera, Indigofera linnaei,
Melilotus indicus, and Pongamia pinnata were collected from the
Amer region and investigated for nodule characteristics and microsymbiont
diversity. Significant interspecific differences were observed in nodulation
patterns. P. pinnata exhibited the highest nodule number (51.33 ± 4.16
nodules/plant) and largest nodules (10 mm), whereas I. hochstetteri
produced the fewest nodules (12.33 ± 2.52). Nodule distribution also varied,
with collar nodules were present only in A. vaginalis and P. pinnata.
Secondary/tertiary roots consistently formed the highest number of nodules
across all species. Morphologically, A. vaginalis formed determinate
nodules with spherical shape, while other plants developed elongated, branched
indeterminate nodules. Rhizobial isolates displayed distinct colony appearances
on YEMA plate, reflecting host-specific associations and exopolysaccharide
production differences. All tested strains showed significant variation in RAPD
pattern obtained from with RPO1-PCR. The isolate from P. pinnata showed
the greatest polymorphism, indicating higher genomic diversity, while A.
vaginalis exhibited fewer bands. These results emphasize the ecological
importance of host-dependent nodule characteristics and microsymbiont diversity
in legume-rhizobia interactions. These findings highlight the effectiveness of
RAPD markers as a rapid tool for genetic profiling of rhizobial populations,
providing valuable insights beyond phenotypic characterization. Moreover, the
results emphasize the ecological importance of perennial legumes such as Pongamia
pinnata, which function as robust nitrogen fixers and play a key role in
sustaining agroforestry systems.
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Pages:9-14
How to cite this article:
Amit Yadav, Sonam Choudhary, Indu Singh Sankhla "Comparative analysis of root nodulation and rhizobial diversity in legume species growing in Amer Region, Jaipur". International Journal of Botany Studies, Vol 10, Issue 10, 2025, Pages 9-14
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