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International Journal of
Botany Studies
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VOL. 10, ISSUE 10 (2025)
Phytochemical Profiling of Ocimum sanctum L. Leaves using GC-MS with Reference to their Pharmaceutical Potential
Authors
Chaitrali R. Hingane, Tejaswini M. Kokate
Abstract
O. sanctum L. (Holy Basil) is a widely used medicinal plant known for its diverse therapeutic properties. In the present study, GC-MS analysis of O. sanctum leaves revealed the presence of twenty primary metabolites, including carbohydrates (glucose, fructose, sucrose), amino acids (alanine, glycine, proline, valine), organic acids (citric, malic, lactic acids), fatty acids (palmitic, stearic, oleic, linoleic acids), sugar alcohols (glycerol, myo-inositol), vitamins, and phytosterols, highlighting its rich biochemical composition. These primary metabolites contribute to energy metabolism, osmoregulation, stress tolerance, antimicrobial activity and neuroprotection. Additionally, twenty-nine secondary metabolites were identified, including monoterpenes (1,8-cineole, camphor, linalool), sesquiterpenes (β-caryophyllene, α-humulene), phenylpropanoids (eugenol, methyl eugenol), flavonoids (apigenin, quercetin, luteolin), polyphenols (rosmarinic acid), and triterpenoids (ursolic acid, oleanolic acid). These compounds are associated with antioxidant, anti-inflammatory, antimicrobial, anticancer, cardio protective, neuroprotective, and metabolic health-promoting activities. The comprehensive GC-MS profiling provides a scientific basis for the traditional medicinal uses of O. sanctum and underscores its potential as a source of bioactive compounds for pharmaceutical and nutraceutical applications.
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Pages:104-108
How to cite this article:
Chaitrali R. Hingane, Tejaswini M. Kokate "Phytochemical Profiling of <i>Ocimum</i> <i>sanctum</i> L. Leaves using GC-MS with Reference to their Pharmaceutical Potential". International Journal of Botany Studies, Vol 10, Issue 10, 2025, Pages 104-108
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