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VOL. 11, ISSUE 1 (2026)
Phytochemical screening and comparative analysis of in vivo and in vitro derived plant parts of Plectranthus amboinicus (Lour.) Spreng.
Authors
Mali Ram Hindala, Vidya Patni
Abstract
Phytochemical screening or characterization is
an important biochemical process for identifying the major phytoconstituents
present in medicinal plants. Plectranthus amboinicus (Indian borage) is
an importantperennial herb that has been valued for its
medicinal properties since ancient times. The goal of the current study was to
evaluate the initial phytochemical makeup of different plant sections (leaves,
roots, and stems) grown under in vivo (naturally grown) and in vitro
(laboratory-grown) conditions. For this purpose, different solvents with
varying polarities, namely water, methanol, acetone, petroleum ether,
chloroform, and hexane were used. Phytochemical screening revealed that
tannins, steroids, terpenoids, and glycosides were present in most of the
extracts and across all plant parts, whereas carbohydrates, proteins,
flavonoids, and phenols were predominantly detected in polar solvents.
Alkaloids and ascorbic acid were found to be absent in all plant parts and
across all extracts. A comparison between in vivo and in vitro
plant parts revealed that in vivo plant parts contained a higher
diversity and abundance of phytochemicals than in vitro plant parts and
callus of Plectranthus amboinicus. The present study suggests that P.
amboinicus is a rich source of phytoconstituents, particularly secondary
metabolites, which may be exploited for future pharmacological and industrial
applications. Although the study was primarily qualitative and intended for
preliminary screening, it provides valuable insight for the targeted extraction
of bioactive compounds and the medicinal utilization of this plant.
Furthermore, the findings establish an important basis for future
investigations aimed at the isolation and characterization of phytoconstituents
from P. amboinicus for the development of novel therapeutic agents.
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Pages:144-152
How to cite this article:
Mali Ram Hindala, Vidya Patni "Phytochemical screening and comparative analysis of <i>in vivo</i> and <i>in vitro</i> derived plant parts of <i>Plectranthus amboinicus</i> (Lour.) Spreng.". International Journal of Botany Studies, Vol 11, Issue 1, 2026, Pages 144-152
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