Myxomycetes, commonly known as plasmodial slime molds, represent a unique group of fungus-like protists that play an important role in nutrient cycling and decomposition in terrestrial ecosystems. The present study was conducted to investigate the biodiversity, habitat preference, and distribution of myxomycetes in the Alibag region of Raigad District, Maharashtra, India. Alibag is a coastal town situated at 18° 38' 29" N latitude and 72° 52' 20" E longitude, on the west coast of India around 300 km south of Mumbai. The average elevation is 0 meters (0 feet). Alibag being a coastal town with temperature range (29 C- 33 C) with average rainfall 2402 mm provide suitable conditions for the occurrence of myxomycetes.