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VOL. 11, ISSUE 1 (2026)
Qualitative and quantitative phytochemistry and antimicrobial activity of Blumea membranacea DC and Blumea oxyodonta DC
Authors
Neha P Kopare, Dipak K Koche
Abstract
Blumea membranacea
DC. and Blumea oxyodonta DC. (Asteraceae) are traditionally used
medicinal plants; however, systematic scientific validation of their
phytochemical composition and antimicrobial potential remains limited. The
present investigation aimed to qualitatively and quantitatively characterize
the phytochemical constituents of root, stem, leaf, and inflorescence and to
evaluate the antimicrobial activity of leaf and inflorescence extracts. Plant
materials were extracted using a Soxhlet apparatus with solvents of increasing
polarity (n-hexane, ethanol, methanol, and distilled water). Preliminary
phytochemical screening revealed the presence of phenolics, tannins, alkaloids,
flavonoids, cardiac glycosides, coumarins, phytosterols, and saponins, while
carbohydrates, proteins, and fatty acids were detected in trace amounts.
Methanolic extracts exhibited the highest extraction efficiency and were
therefore selected for quantitative estimation and antimicrobial assays.
Quantitative analysis demonstrated significantly higher concentrations of alkaloids,
phenolics, flavonoids, tannins, and coumarins in leaf tissues compared with
roots, stems, and inflorescences. Antibacterial activity of methanolic leaf
extracts was evaluated using the agar well diffusion method against selected
Gram-positive and Gram-negative bacterial strains, revealing pronounced
inhibitory effects, whereas no appreciable antifungal activity was observed
against Aspergillus niger and Candida albicans. Inflorescence
extracts exhibited comparatively weak antibacterial activity and lacked
antifungal effects. Gentamicin and ciprofloxacin were used as standard
antibacterial controls, while itraconazole and amphotericin B served as
antifungal standards. Minimum inhibitory concentration (MIC) assays confirmed
significant antibacterial efficacy at low extract concentrations. Collectively,
these findings substantiate the ethnomedicinal relevance of B. membranacea
and B. oxyodonta and highlight their potential as promising sources of
bioactive compounds for the development of novel antimicrobial agents.
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Pages:376-386
How to cite this article:
Neha P Kopare, Dipak K Koche "Qualitative and quantitative phytochemistry and antimicrobial activity of <i>Blumea membranacea </i>DC<i> and Blumea oxyodonta </i>DC". International Journal of Botany Studies, Vol 11, Issue 1, 2026, Pages 376-386
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