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VOL. 11, ISSUE 2 (2026)
Molecular identification and phylogenetic characterization of Sesamum indicum L. using ITS DNA barcoding
Authors
Jotiram D Salunke, Dr. Gulab M Rathod, Srushti S Kale
Abstract
Accurate molecular
identification is essential for plant taxonomy, conservation, and
pharmacological applications. The present study aimed to authenticate Sesamum
indicum L. using Internal Transcribed Spacer (ITS) DNA barcoding and to
evaluate its phylogenetic relationships with related taxa. Genomic DNA was
extracted from leaf samples and sequenced using Sanger sequencing. The obtained
ITS sequence (~452 bp) was analyzed using BLAST, which showed 99.34% similarity
with Sesamum indicum (GenBank accession: KM210317.1), confirming species
identity. Phylogenetic analysis was performed using the Neighbor-Joining method
with bootstrap support (500 replicates), revealing clustering of the sample
with S. indicum and clear divergence from related genera. The genetic
distance matrix further supported minimal intraspecific divergence (0.0067) and
significant interspecific variation. The results demonstrate that ITS markers
are reliable tools for molecular identification and phylogenetic analysis of Sesamum
indicum L.
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Pages:23-28
How to cite this article:
Jotiram D Salunke, Dr. Gulab M Rathod, Srushti S Kale "Molecular identification and phylogenetic characterization of <i>Sesamum indicum</i> L. using ITS DNA barcoding". International Journal of Botany Studies, Vol 11, Issue 2, 2026, Pages 23-28
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