ARCHIVES
VOL. 11, ISSUE 2 (2026)
Callogenesis in Hemidesmus indicus (L.): Optimization of In vitro culture conditions for medicinal plant propagation
Authors
Siddhant Rajendrarao Dhokane
Abstract
Hemidesmus indicus
(L.), an important medicinal plant widely used in traditional systems of
medicine, faces challenges in large-scale propagation and conservation. The
present study focuses on the induction and optimization of callus formation
(callogenesis) using different explants (leaf, nodal, and petiole) under in
vitro conditions. Murashige and Skoog (MS) medium supplemented with varying
concentrations and combinations of plant growth regulators (2,4-D, NAA, BAP,
and IBA) was employed. Among all explants, leaf segments exhibited the highest
callogenic response (100%) on MS medium supplemented with 2,4-D (1.5 mg/L) and
NAA (1.5 mg/L). Callus initiation was observed within 9–12 days of inoculation.
The study demonstrates that optimized hormonal combinations significantly
influence callus induction efficiency. The developed protocol can be utilized
for large-scale propagation, conservation, and secondary metabolite production
in H. indicus.
Download
Pages:16-22
How to cite this article:
Siddhant Rajendrarao Dhokane "Callogenesis in <i>Hemidesmus indicus</i> (L.): Optimization of <i>In vitro</i> culture conditions for medicinal plant propagation". International Journal of Botany Studies, Vol 11, Issue 2, 2026, Pages 16-22
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.