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VOL. 11, ISSUE 2 (2026)
Investigation of proline accumulation in stress-induced and normal plants of trigonella Foenum-Graecum: A comparative analysis of abiotic stress responses
Authors
Pratiksha Ramesh Himane
Abstract
Abiotic stress represents a major
environmental constraint affecting plant growth, metabolic homeostasis, and
agricultural productivity worldwide. The present investigation aimed to
evaluate proline accumulation in Trigonella foenum-graecum subjected to different
abiotic stress conditions, including salinity, water deficit, and light
deprivation, in comparison to normal growth conditions. Proline quantification
was conducted using the acid ninhydrin spectrophotometric assay at 520 nm.
Experimental findings revealed the highest proline accumulation under dark
stress (0.232 µmoles g⁻¹ tissue), followed by water stress (0.167 µmoles g⁻¹
tissue), salt stress (0.155 µmoles g⁻¹ tissue), and control conditions (0.093
µmoles g⁻¹ tissue). Morphological observations further indicated significant
alterations in plant height, root elongation, and foliar development under
stress treatments. The pronounced proline accumulation under dark stress
suggests that light deprivation may serve as a potent inducer of osmoprotective
metabolism in fenugreek. The study establishes proline as a reliable
biochemical indicator of abiotic stress and highlights dark stress as a
comparatively underexplored physiological stressor influencing plant adaptive
metabolism.
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Pages:147-151
How to cite this article:
Pratiksha Ramesh Himane "Investigation of proline accumulation in stress-induced and normal plants of trigonella Foenum-Graecum: A comparative analysis of abiotic stress responses". International Journal of Botany Studies, Vol 11, Issue 2, 2026, Pages 147-151
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