Alveolar lung adenocarcinoma is the most prevalent form of cancer that leads to high mortality worldwide. The conventional chemotherapies affect human health and life in a disruptive way. To combat these, nanoatechnology stood as a promising solution with efficacy, also the green synthesis adds the potential to the nanoparticles with their phytochemicals. Hence, the present study was designed to synthesize the silver nanoparticles from Senna Montana – leaf, bark and fruit extracts and evaluate their anticancerous activtiy against A549 cell lines through MTT assay. The biosynthesized silver nanoparticles (SNPs) from various parts exhibited varying degrees of antiproliferative activity. The fruit-mediated SNPs (SmF) showed highest cytotoxic potential with an IC₅₀ value of 32.1 µg/mL, followed by bark-derived SNPs (SmB) with an IC₅₀ of 70.78 µg/mL. But, SNPs from leaf extract exhibited lower activity with an IC₅₀ value of 95.08 µg/mL. The results indicate that Senna montana-mediated AgNPs, particularly those synthesized from fruit extracts, possess significant anticancer activity against A549 cells.