ARCHIVES
VOL. 11, ISSUE 2 (2026)
Molecular systematics, DNA barcoding and conservation genetics of Anogeissus Guill. (DC.) & Perr. in the expanded genus Terminalia L. A review with special reference to Indian Arid-Zone taxa (Combretaceae)
Authors
Ravi Kant Saini, Himanshu Sisodia, Karan Singh Mororiya, Jai Singh
Abstract
This review summarises recent advances on
molecular systematics, DNA barcoding and conservation genetics of Anogeissus
(DC.) Guill. & Perr. in the extended genus Terminalia L.
(Combretaceae). Recent phylogenetic and phylogenomic studies have consistently
placed genus Anogeissus within Terminalia resulting in a major
taxonomic rearrangement of the group. The molecular basis of this
reclassification is reviewed and its implications for species delimitation and
conservation of Indian arid zone taxa are discussed. Methodological aspects of
molecular studies in the Combretaceae with special reference to Phylogeny of Anogeissus
and Terminalia and DNA extraction from polyphenol- and
polysaccharide-rich tissues. Studies have demonstrated that recovery of
PCR-amplifiable DNA from mature tree tissues requires an optimised three-step
extraction protocol comprising a pre-washing with Triton X-100, modified CTAB
extraction with polyvinylpyrrolidone and ascorbic acid, and spin-column
purification. Comparative analysis of the barcode loci (rbcL, matK,
ITS and psbA-trnH) suggests that matK provides higher species level
resolution and that multilocus approaches are more powerful than single marker
analysis. Anogeissus species are ecologically and medicinally important
but we have very little knowledge about the genetic diversity and population
structure of these species. The review suggests to combine multilocus DNA
barcoding with highly polymorphic markers (e.g. ISSRs and microsatellites) for
better conservation genetics and species delimitation studies.
Download
Pages:348-357
How to cite this article:
Ravi Kant Saini, Himanshu Sisodia, Karan Singh Mororiya, Jai Singh "Molecular systematics, DNA barcoding and conservation genetics of <i>Anogeissus</i> Guill. (DC.) & Perr.<i> </i>in the expanded genus <i>Terminalia</i> L.<i> </i>A review with special reference to Indian Arid-Zone taxa (Combretaceae)". International Journal of Botany Studies, Vol 11, Issue 2, 2026, Pages 348-357
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.