Maintenance of soil organic matter is very much important for the long-term productivity of agro ecosystems. Legume crop (Vigna radiata L Wilczek. Var CO4) was cultivated to overcome the growing problem of nitrogen fixation of plants grown in pesticides polluted soils. To improve the soil quality for the effective growth of green gram the use of microorganism for bioremediation of organic pollutants was done. Pot culture experiment was performed and the effect of pesticides with Bradyrhizobium japonicum, Bacillus megaterium, and Bacillus mucilaginous was analysed and mineral content of V. radiata L. were observed, studied at various stages of its growth. The morphometric analysis, yield parameters were calculated individually in each pot on 30, 40, 60 DAS. The control pot was characterized by a stronger growth, more abundant green matter, and developed root system with more nodules. Among all treatments the combined inoculation of bio-fertilizers recorded the highest growth and yield of green gram 24.779(mg/ml), inoculation of biofertilizer Bradyrhizobium japonicum only yielded with a protein mass of 23.715 (mg/ml). Bradyrhizobium japonicum favoured a considerable growth, yield for leguminous plants in germination of pot culture experiments that is helpful for development of plant communities and eco-friendly environment.