ARCHIVES
VOL. 6, ISSUE 5 (2021)
Evaluation of phytochemical constituents and antimicrobial activity of a novel polyherbal formulation
Authors
Fouzia Tehseen, Syed Safiullah Ghori, Ruqiya Khatoon, Qurrath Ul Ain Sana, Syed Mustafa Fawal
Abstract
The present study was performed for evaluation of antimicrobial activity of a novel polyherbal formulation(PHF) containing plants viz. Piper longum, Smilax china, Swertia chirata and Withania somnifera against bacteria (gram positive bacteria: S.aureus, B.subtilis and gram negative bacteria: E.coli and P.aeroginosa) and fungi(A.niger and C.albicans) responsible for various infections. The PHF was extracted using petroleum ether and ethanol to get three different concentrations of 25 µg/ml, 50 µg/ml and 100 µg/ml of each. Evaluation of phytochemical constituents revealed the presence of secondary metabolites like sugars, alkaloids, steroids, glycosides, tannins, saponins and flavonoids. Both the extracts were evaluated for antibacterial and antifungal activity by well diffusion assays. Ciprofloxacin and Miconazole were used as positive controls for antibacterial and antifungal activity respectively. All the extracts showed moderately significant antimicrobial activity. The 100 µg/ml ethanolic extract of the PHF was most effective against Candida albicans showing 30 mm zone of inhibition. The antimicrobial potency of the PHF may be due to the presence of tannins and polyphenols like flavonoids. The PHF showed synergistic broad spectrum antimicrobial activity providing scope for dosage formulation and further investigation for other pharmacological activities.
Download
Pages:1288-1291
How to cite this article:
Fouzia Tehseen, Syed Safiullah Ghori, Ruqiya Khatoon, Qurrath Ul Ain Sana, Syed Mustafa Fawal "Evaluation of phytochemical constituents and antimicrobial activity of a novel polyherbal formulation ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 1288-1291
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.