Alternanthera bettzeckiana also known as calico plant belongs to Amaranthaceae family. The present study was designed to carry out the phytochemical screening of the different extracts of the leaves of Alternanthera bettzeckiana and identify the bioactive compounds present in the acetone extract using GC MS analysis. A total of 11 compounds were identified from the acetone extract of leaves. The main compounds identified are Phytol, 3, 7, 11, 15-tetramethyl-2-hexadecen-1-ol, Geranyl geraniol and Vitamin E.