Lawsonia inermis (Henna) is a cosmetic dye. Henna leaves were extensively used as dye for nails, hands, hair and textile and also used in treating certain skin infections, headache, jaundice, amebiasis and enlargement of the spleen. In the present study an attempt was made to evaluate the anti-asthmatic activity of ethanolic extract of Lawsonia inermis (EELI) leaves against ovalbumin (OVA) -induced asthma in albino mice. The parameters assessed were evaluation of lung inflammation, OVA-specific immunoglobulin E titre by ELISA and histopathology of lung. EELI at the dose range of 100 and 200 mg/kg i.p inhibited ovalbumin induced asthma by decreasing the release of inflammatory mediators. The present study indicates that Lawsonia inermis has significant anti-asthmatic property.