Now a days, synthetic dyes became serious health hazard as it releases vast amount of waste and unfixed colorants and are troubling the eco-balance of the nature. Due to environmental matters in the production besides application of synthetic dyes, now-a-days consumers interest in natural dyes ones again revived. The present study expresses dyeing of cotton cloth using natural dye obtained from the floral parts of Mirabilis jalapa Linn. Pre-mordanting, application of dye to cotton with and without using mordant as well as at different pH was tried. The pre-mordanting was performed by using four chosen mordants i.e., Ferrous sulphate, Potassium dichromate, Copper sulphate, Tannic acid and their combinations. Separate mordants and their blends showed different colour shades. The dyeing developed various shades of brown, black, khaki and pink colour. The colour developed and their intensity depends on the type of mordants and respective pH conditions. Among them Ferrous sulphate and Tannic acid impart darker shades. As the pH of dye bath increases, the colour intensifies and the pH 8 offered improved colour shades as compared to pH 4. Ferrous sulphate and Tannic acid were found to be more suitable among the used mordants, as they exhibited better dyeing output.