The objective of this study was to isolate endophytic bacteria from the stem of Leptadenia pyrotechnica, their identification and investigation of their morphological characterisation. Fresh stem of Leptadenia pyrotechnica was procured from the department of botany, university of Rajasthan.

Endophytes are harmless organisms that reside in the tissues of their hosts. Their tight interaction causes them to develop secondary metabolites. It is thought that the bioactive substances found in endophytes cause less damage to normal cells. It is important to investigate endophytes and related compounds. The isolates' cultural traits on nutrient agar petriplates and slants differed greatly. On nutrient agar petriplates, the bacterial colonies had a creamy white appearance, a circular shape, and uneven or complete colony edges. The isolates processed different sources of carbohydrates. One isolate were tested gram negative and fifteen gram positive.

Leptadenia pyrotechnica is a remarkable desert shrub whose structural adaptations ensure survival in harsh, arid environments, while its extensive ethno botanical use and rich phytochemical composition reinforce its significant therapeutic and ecological value. Whether viewed through the lens of traditional medicine, environmental restoration, or drug discovery, it stands as a plant of considerable promise.

Our findings especially the strong Gram-positive dominance and unusual prevalence of Streptococcus largely align with patterns seen in desert plant endophytes, reinforcing the adaptability and environmental resilience of such communities. The disparity compared to more diverse medicinal plant systems highlights the uniqueness of L. pyrotechnica’s micro biome. The endophytes having circular forms 68.75%, entire margins 25%, convex elevation 37.50% large size character 81.25% predominated among all the isolates on nutrient agar plates. The endophytes having bacterial growth on nutrient agar slant moderate growth 68.75%, opaque optical character 25%, mucoid consistency 37.50%, filliform form 81.25% predominated among all the isolates.