Sacred Groves are the house of indigenous ancestor plants, which are often found abundant in the area. Many local communities rely on these groves as a source of traditional knowledge, utilizing this knowledge for treating a variety of ailments. The study examines the cultural, religious, and ecological significance of sacred groves in Gujarat's Mangrol, Mandvi, and Umarpada talukas, emphasizing their crucial role in conserving forests and preserving plant biodiversity. Documenting 382 plant species and 50 ethno-medicinal plants, the research underscores the need for urgent conservation efforts and community involvement. The study highlights the importance of interdisciplinary collaboration and education initiatives for the sustainable management and preservation of these culturally and ecologically significant sacred groves.